1: Integr Cancer Ther. 2007 Sep;6(3):271-80.
Hepatoprotective effect of andrographolide against hexachlorocyclohexane-induced oxidative injury.
Trivedi NP, Rawal UM, Patel BP.
Department of Zoology, School of Sciences, Gujarat University, Ahmedabad, India.
Many plant products are known to exert antioxidative effects by quenching various free radicals and singlet molecular oxygen. Andrographis paniculata (Kalmegh) is used extensively in the Indian traditional system of medicine as a hepatoprotective and hepatostimulative agent and has been reported to have antioxidant effects against different hepatotoxins. The present study aims to analyze antioxidant properties of an active component, andrographolide (ANDLE), extracted from A paniculata. This study investigates the effect of andrographolide on the hepatocellular antioxidant defense system and lipid peroxidation of control mice, mice treated with hexachlorocyclohexane (BHC) only, and andrographolide + BHC. Glutathione (GSH), glutathione-s-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GSH-Px), gamma-glutamyl transpeptidase (gamma-GTP), superoxide dismutase (SOD), catalase (CAT), and lipid peroxidation (LPO) are studied by spectrophotometric methods. The BHC experimental model forms an irreversible liver tumor in male mice. The activities of GSH, GR, GSH-Px, SOD, and CAT show significant (P </= .05) increases, while gamma-GTP and GST show significant decreases (P </= .05) in andrographolide-supplemented mice as compared with BHC-treated mice. This study indicates that the antioxidant effect of andrographolide could be due to its ability to activate antioxidant enzymes that catalyze the reaction of oxidants and are effective in severe liver damage.
PMID: 17761640 [PubMed - in process]
2: Phytother Res. 2007 Aug 17; [Epub ahead of print] Related Articles, LinkOut
Hepatoprotective effect of leaf extracts of Andrographis lineata nees on liver damage caused by carbon tetrachloride in rats.
Sangameswaran B, Reddy TC, Jayakar B.
Vinayaka Mission's College of Pharmacy, Vinayaka Missions University, K.N. Patty, Salem-636 008, Tamil Nadu, India.
The present study was conducted to evaluate the hepatoprotective effect of Andrographis lineata (Acanthaceae) extracts in carbon tetrachloride-induced liver injury in rats. Male Wistar rats with chronic liver damage, induced by subcutaneous injection of 50% v/v carbon tetrachloride in liquid paraffin at a dose of 3 mL/kg on alternate days for a period of 4 weeks, were treated with methanol and aqueous extracts of A. lineata orally at a dose of 845 mg/kg/day. The biochemical parameters such as serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum bilirubin and alkaline phosphatase were estimated to assess the liver function. Histopathological studies of the liver were also carried out to confirm the biochemical changes. Histopathological examinations of liver tissue corroborated well with the biochemical changes. The activities of extracts were comparable to a standard drug. Hepatic steatosis, hydropic degeneration and necrosis were observed in the carbon tetrachloride treated group, while these were completely absent in the standard and extract treated groups. A. lineata extracts exhibited hepatoprotective action against carbon tetrachloride-induced liver injury. The present investigation established pharmacological evidence to support the folklore claim that it is used traditionally as a hepatoprotective agent. Copyright (c) 2007 John Wiley & Sons, Ltd.
PMID: 17705141 [PubMed - as supplied by publisher]
3: Anticancer Res. 2007 Jul-Aug;27(4B):2439-47. Related Articles, LinkOut
Andrographolide inhibits the adhesion of gastric cancer cells to endothelial cells by blocking E-selectin expression.
Jiang CG, Li JB, Liu FR, Wu T, Yu M, Xu HM.
Department of Oncology, The First Affiliated Hospital, Shenyang, Liaoning Province, China.
BACKGROUND: Andrographolide, an active component isolated from the Chinese official herbal Andrographis paniculata, has recently been reported to have anticancer activity. However the molecular mechanism responsible for its anticancer action has not been fully defined. In this study, we investigated the effect of andrographolide on the adhesion of gastric cancer cells to the activated endothelial cells and the expression of some cell adhesion molecules. MATERIALS AND METHODS: Human endothelial cells were preincubated with andrographolide for 6 h and then incubated with the cytokine tumor necrosis factor for 4 h. Endothelial surface expression of E-selectin was evaluated by flow cytometry, immunostaining and ELISA. Further, we investigated E-selectin mRNA expression by RT-PCR. Surface expression of sialyl Lewis(X) of three gastric cancer cell lines (SGC7901, MGC803, BGC823) and a normal gastric epithelial cell line GES-1 was evaluated by flow cytometry and immunostaining. Adherence of CFSE-labeled gastric cancer cells and GES-1 cells to endothelial cell monolayers was then determined. RESULTS: Andrographolide significantly reduced E-selectin expression of activated endothelial cells, and inhibited the E-selectin expression on mRNA level. Three gastric cancer cell lines expressed high levels of sialyl Lewis(X), whereas GES-1 did not. Andrographolide also significantly decreased gastric cancer cells adherence to stimulated endothelial cells. The inhibition of E-selectin expression corresponded to the reduction of tumor cell adherence. The effects of andrographolide on tumor adhesion were almost nullified by pre-incubation with E-selectin and sialyl Lewis(X) antibody. CONCLUSION: These findings demonstrate that andrographolide suppresses the adhesion of gastric cancer cells which express high level sialyl Lewis(X) to human vascular endothelial cells by blocking E-selectin expression and, thus, may represent a candidate therapeutic agent for cancer.
PMID: 17695536 [PubMed - in process]
4: Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(12):1015-7. Related Articles, Compound via MeSH, Substance via MeSH, LinkOut
[Effect of andrographolide on QS regulating virulence factors production in Pseudomonas aeruginosa]
[Article in Chinese]
Li HT, Qin HM, Wang WH, Li GJ, Wu CM, Song JX.
Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
OBJECTIVE: To investigate the effect of andrographolide on virulence factors production in Pseudomonas aeruginosa. METHOD: Growth rate, pyocyanin, proteolytic activity and elastase activity were measured with or without the presence of andrographolide. The effect of andrographolide on pyocyanin production, proteolytic activity and elastase activity in PAO-JP2 was investigated simultaneously. RESULT: The andrographolide did not affect the growth of PAO1 in planktonic culture. The production of pyocyanin, proteolytic activity and elastase activity were significanthy suppressed in P. aeruginosa cultures grown in the presence of andrographolide. However, these effects were not observed in PAO-JP2. CONCLUSION: The inhibiting effect of andrographolide on virulence factors production in P. aeruginosa may play a role in its anti-infection activity.
Publication Types:- English Abstract
- Research Support, Non-U.S. Gov't
PMID: 17048654 [PubMed - indexed for MEDLINE]
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