Inhibitory effects of ethyl acetate extract of Andrographis paniculata
on NF-{kappa}B trans-activation activity and LPS-induced acute
inflammation in mice
Chao WW, Kuo YH, Hsieh SL, Lin BF.
Department of Biochemical Science and Technology, Institute of
Microbiology and Biochemistry, College of Life Science, National
Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617,
Taipei, Taiwan, ROC. bifong@ntu.edu.tw.
This study was to investigate anti-inflammatory effect of Andrographis
paniculata (Burm. f.) Nees (Acanthaceae) (AP). The effects of ethyl
acetate (EtOAc) extract from AP on the level of inflammatory mediators
were examined first using nuclear factor kappa B (NF-kappaB) driven
luciferase assay. The results showed that AP significantly inhibited
NF-kappaB luciferase activity and tumor necrosis factor alpha
(TNF-alpha), interleukin 6 (IL-6), macrophage inflammatory protein-2
(MIP-2) and nitric oxide (NO) secretions from lipopolysaccharide
(LPS)/interferon-gamma stimulated Raw264.7 cells. To further evaluate
the anti-inflammatory effects of AP in vivo, BALB/c mice were tube-fed
with 0.78 (AP1), 1.56 (AP2), 3.12 (AP3) and 6.25 (AP4) mg kg(-1) body
weight (BW)/day in soybean oil, while the control and PDTC
(pyrrolidine dithiocarbamate, an anti-inflammatory agent) groups were
tube-fed with soybean oil only. After 1 week of tube-feeding, the PDTC
group was injected with 50 mg kg(-1) BW PDTC and 1 h later, all of the
mice were injected with 15 mg kg(-1) BW LPS. The results showed that
the AP1, AP2, AP3 and PDTC groups, but not AP4, had significantly
higher survival rate than the control group. Thus, the control, AP1,
AP2, AP3 and PDTC groups were repeated for in vivo parameters. The
results showed that the AP and PDTC groups had significantly lower
TNF-alpha, IL-12p40, MIP-2 or NO in serum or peritoneal macrophages
and infiltration of inflammatory cells into the lung of mice. The AP1
group also had significantly lower MIP-2 mRNA expression in brain.
This study suggests that AP can inhibit the production of inflammatory
mediators and alleviate acute hazards at its optimal dosages.
PMID: 19745004 [PubMed - as supplied by publisher]
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