J Ethnopharmacol. 2010 Mar 19. [Epub ahead of print]
Chandrasekaran CV, Gupta A, Agarwal A.
Department of Cellular Assay, R & D centre, Natural Remedies Pvt. Ltd., Bangalore, India.
AIM OF STUDY: Andrographis paniculata has been known to possess widespread traditional application in the treatment of allergy and inflammatory diseases. In the current study, we sought to examine the effects of an extract of Andrographis paniculata leaves on inhibition of lipopolysaccharide (LPS) induced [nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin-1beta (IL-1 beta), and interleukin-6 (IL-6)] and calcimycin (A23187) induced [leukotriene B(4) (LTB(4)), thromboxane B(2) (TXB(2)) and histamine] mediators in diverse cell based models. MATERIALS AND METHODS: Effect of an extract of Andrographis paniculata leaves (AP) was studied on inhibition of LPS induced NO, PGE(2), IL-1 beta and IL-6 in J774A.1 murine macrophages; A23187 induced LTB(4) and TXB(2) in HL-60 promyelocytic leukemic cells and histamine in RBL-2H3 rat basophilic leukemia cells. RESULTS AND CONCLUSION: AP illustrated significant alleviation of pro-inflammatory, inflammatory, and allergic mediators. However, no inhibition was observed against histamine release. This outcome has been summed up to deduce that AP is fairly potent in attenuating the inflammation by inhibiting pro-inflammatory (NO, IL-1 beta and IL-6), inflammatory (PGE(2) and TXB(2)) and allergic (LTB(4)) mediators. Copyright © 2010. Published by Elsevier Ireland Ltd.
MID: 20307638 [PubMed - as supplied by publisher]
A new diterpene from the leaves of Andrographis paniculata Nees
Fitoterapia. 2010 Mar 12. [Epub ahead of print]
Xu C, Chou GX, Wang ZT.
The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, P. R. China.
Phytochemical investigation of the ethanol extract of the leaves of Andrographis paniculata yielded one novel diterpene (13R, 14R) 3, 13, 14, 19-tetrahydroxy-ent-labda-8 (17), 11-dien-16, 15-olide 1 which has an uncommon cis-diol groups in the lactone moiety, and 3, 19-isopropylidene-14-deoxy-ent-labda-8 (17), 13-dien-16, 15-olide 2, probably an artifact diterpene, together with eight known diterpenoids 3-10. The structures of these compounds were determined on the basis of spectral methods. The structure and stereochemistry of 1 was confirmed by X-ray crystallographic analyses. Copyright © 2010 Elsevier B.V. All rights reserved.
PMID: 20230876 [PubMed - as supplied by publisher]
Andrographolide regulates epidermal growth factor receptor and transferrin receptor trafficking in epidermoid carcinoma (A-431) cells
Br J Pharmacol. 2010 Mar 3. [Epub ahead of print]
Tan Y, Chiow KH, Huang D, Wong SH.
Tan Y, Chiow KH, Huang D, Wong SH.
Laboratory of Membrane Trafficking and Immunoregulation, Department of Microbiology, Immunology Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore.
Background and purpose: Andrographolide is the active component of Andrographis paniculata, a plant used in both Indian and Chinese traditional medicine, and it has been demonstrated to induce apoptosis in different cancer cell lines. However, not much is known about how it may affect the key receptors implicated in cancer. Knowledge of how andrographolide affects receptor trafficking will allow us to better understand new mechanisms by which andrographolide may cause death in cancer cells. Experimental approach: We utilized the well-characterized epidermal growth factor receptor (EGFR) and transferrin receptor (TfR) expressed in epidermoid carcinoma (A-431) cells as a model to study the effect of andrographolide on receptor trafficking. Receptor distribution, the total number of receptors and surface receptors were analysed by immunofluorescence, Western blot as well as flow-cytometry respectively. Key results: Andrographolide treatment inhibited cell growth, down-regulated EGFRs on the cell surface and affected the degradation of EGFRs and TfRs. The EGFR was internalized into the cell at an increased rate, and accumulated in a compartment that co-localizes with the lysosomal-associated membrane protein in the late endosomes. Conclusion and implications: This study sheds light on how andrographolide may affect receptor trafficking by inhibiting receptor movement from the late endosomes to lysosomes. The down-regulation of EGFR from the cell surface also indicates a new mechanism by which andrographolide may induce cancer cell death.
PMID: 20233216 [PubMed - as supplied by publisher]
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