Evaluation of immunomodulatory activity of an extract of
andrographolides from Andographis paniculata.
Naik SR, Hule A.
Prin. K. M. Kundnani College of Pharmacy, Rambhau Salgaonkar Marg,
Cuff, Parade, Colaba, Mumbai, India. srnaik5@rediffmail.com
The immunomodulatory activity of HN-02, an extract containing a
mixture of andrographolides (i.e., andrographolide [88 +/- 5 %] plus
14-deoxyandrographolide and 14-deoxy-11,12-didehydroandrographolide
together [12 +/- 3 %]) in a pure powder form was evaluated at 1.0,
1.5, and 2.5 mg/kg on different in vivo and in vitro experimental
models. In a delayed-type hypersensitivity (DTH) mouse model,
potentiation of the DTH reaction was observed after treatment with
cyclophosphamide (CYP) and HN-02 individually. However, CYP
potentiation of the DTH reaction was reversed by HN-02 pretreatment.
Furthermore, HN-02 treatment elevated the depressed hemagglutination
antibody (HA) titer and increased the number of plaque-forming cells
(PFCs) in the spleen cells of mice that had been treated with CYP and
challenged with sheep red blood cells (SRBC). Further, it was also
found that HN-02 treatment stimulated phagocytosis in mice. A
significant increase in total WBC count and relative weight of spleen
and thymus was observed in mice during 30 days of treatment with
HN-02. The present experimental findings demonstrate that HN-02 has
the ability to enhance immune function, possibly through modulation of
immune responses altered during antigen interaction, and to reverse
the immunosuppression induced by CYP.
PMID: 19263340 [PubMed - indexed for MEDLINE]
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