Chemical Fingerprinting of Andrographis paniculata (Burm. f.) Nees by
HPLC and Hierarchical Clustering Analysis.
Dong HJ, Zhang ZJ, Yu J, Liu Y, Xu FG.
Key laboratory of Drug Quality Control and Pharmacovigilance (China
Pharmaceutical University), Ministry of Education, Nanjing
210009,China; Center for Instrumental Analysis, China Pharmaceutical
University, Nanjing 210009, China.
The aim is to develop a simple and specific method for the extraction
and chemical fingerprinting of Andrographis paniculata (Burm. f.) Nees
and to apply the method to this drug from different regions.
High-performance liquid chromatographic (HPLC) with gradient elution
is used for developing the fingerprints, and liquid chromatography
electrospray ionization mass spectrometry (LC-ESI-MS) technique is
employed to identify the component of the fingerprints. Nine peaks are
selected as common peaks, and six compounds are elucidated by MS data.
Twenty-three samples of A. paniculata from different regions of China
are collected and detected by HPLC fingerprinting. Comparisons of the
chromatograms show that there are obvious differences in the content
of each component contained between the habitat samples in China. The
results of hierarchical cluster analysis show that these samples can
be clustered reasonably into three groups, and the growth of A.
paniculata and their internal quality are related to their habitat.
The HPLC fingerprint developed allows simple identification of A.
paniculata from many natural drugs.
PMID: 19930808 [PubMed - in process]
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