Behera PR, Nayak P, Barik DP, Rautray TR, Thirunavoukkarasu M, Chand PK.
Plant Cell and Tissue culture Facility, Post-Graduate Department of
Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Orissa,
India; Plant Biotechnology Lab, Institute of Minerals & Materials
Technology (CSIR), Bhubaneswar 751013, Orissa, India.
The multi-elemental composition of in vitro-proliferated root tissues
of Andrographis paniculata (Burm.f.) Wall. ex Nees was compared with
that of the naturally grown in vivo plants. Trace elements namely Cr,
Mn, Fe, Co, Ni, Cu, Zn, Se, Rb, Sr and Pb in addition to two
macro-elements K and Ca were identified and quantified in root tissues
of both sources using the energy dispersive X-ray fluorescence
(ED-XRF) technique. ED-XRF analysis was performed using Mo K X-rays
generated from a secondary molybdenum target. The elemental content of
in vitro roots was found to be at par with that of naturally grown
plants of the same species. This opens up a possibility of exploiting
in vitro root cultures as a viable, alternative and renewable source
of phytochemicals of relevance, besides providing a means for
conservation of the valuable natural resources. Copyright © 2010
Elsevier Ltd. All rights reserved.
PMID: 20637644 [PubMed - as supplied by publisher]
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