Studies on effects of indigenous plant extracts on malarial vector, Anopheles subpictus Grassi (Diptera:Culicidae)

Trop Biomed. 2010 Aug;27(2):143-54.
Studies on effects of indigenous plant extracts on malarial vector, Anopheles subpictus Grassi (Diptera:Culicidae).
Elango G, Abdul Rahuman A, Bagavan A, Kamaraj C, Abduz Zahir A, Rajakumar G, Marimuthu S, Santhoshkumar T.

Unit of Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore District, Tamil Nadu, India.

Abstract

Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The present investigations were made to evaluate the repellent, ovicidal and oviposition-deterrent potential of leaf hexane and chloroform extracts of Aegle marmelos, Andrographis lineata, Andrographis paniculata, Cocculus hirsutus, Eclipta prostrata and Tagetes erecta against Anopheles subpictus Grassi (Diptera:Culicidae). The hexane extract of A. lineata was more effective in exhibiting the repellent action against the mosquito as compared with A. marmelos extract. Complete protections for 150 min were found in hexane extract of A. lineata at 500 ppm against An. subpictus bites. Mean percent hatchability of the ovicidal activity was observed 24 h after treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. No hatchability was observed with hexane and chloroform extracts of A. lineata, A. paniculata and hexane extract of T. erecta were exerted at 1,000 ppm. The percentage of effective oviposition repellency of 93.07, 93.95, 98.03, 90.43, 92.63, 81.53, 94.81, 97.50, 97.26, 92.22, 82.85 and 72.77 at 500 ppm and the lowest repellency of 62.03, 53.64, 73.47, 59.05, 57.95, 48.17, 62.22, 72.99, 75.48, 67.77, 40.57 and 52.11 at 31.25 ppm in hexane and chloroform extracts of A. marmelos, A. lineata, A. paniculata, C. hirsutus, E. prostrata and T. erecta, respectively. The oviposition activity index (OAI) values revealed that the solvent plant extracts have deterrent effect, and they caused a remarkable negative response resulting in oviposition of very few eggs. These results clearly reveal that the hexane extract of A. lineata, served as a potential repellent, ovicidal and oviposition- deterrent against An. subpictus.

PMID: 20962710 [PubMed - in process]