Chao HP, Kuo CD, Chiu JH, Fu SL.
Institute of Traditional Medicine, National Yang-Ming University,
Taipei, Taiwan.
Andrographolide, a major constituent of ANDROGRAPHIS PANICULATA, was
previously shown to exhibit anti-inflammatory, antiviral, and
anticancer activities. The anticancer activity of andrographolide
includes growth suppression, apoptosis promotion, antiangiogenesis,
and antitransformation. However, the effect of andrographolide on
cancer metastasis, the most malignant feature of cancer, has not been
elucidated extensively. In the present study, we demonstrated that
andrographolide at nontoxic to subtoxic concentrations (0.3-3 microM)
suppressed the invasion ability of CT26 cells in Matrigel-based
invasion assays. In addition, the expression of cell adhesion
regulators ( beta-catenin and ILK) was not altered by andrographolide
treatment. However, andrographolide indeed inhibited matrix
metalloproteinase 2 (MMP2) activity without affecting its expression.
Furthermore, the activation of ERK, but not Akt, was attenuated by
andrographolide treatment. Notably, a similar inhibitory effect of
andrographolide on the invasion and MMP2 activity of the human colon
cancer cell line HT29 was also observed. In summary, our results
indicate that andrographolide exhibits anti-invasive activity against
colon cancer cells via inhibition of MMP2 activity. © Georg Thieme
Verlag KG Stuttgart · New York.
PMID: 20539971 [PubMed - as supplied by publisher]
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